Hypertonic Saline vs Normal Saline in Bronchiolitis

3% Hypertonic Saline Versus Normal Saline in Inpatient Bronchiolitis: A Randomized Controlled Trial

BACKGROUND AND OBJECTIVES: We aimed to investigate the effect of nebulized 3% Hypertonic Saline compared with nebulized normal saline (NS) on length of stay (LOS) in infants hospitalized with bronchiolitis. Bronchiolitis, the most common reason for hospitalization in children younger than 1 year in the United States, has no proven therapies effective beyond supportive care.

METHODS: We conducted a prospective, randomized, double-blind, controlled trial in an urban tertiary care children’s hospital in 227 infants younger than 12 months old admitted with a diagnosis of bronchiolitis (190 completed the study); 113 infants were randomized to Hypertonic Saline (93 completed the study), and 114 to NS (97 completed the study). Subjects received 4 mL nebulized 3% Hypertonic Saline or 4 mL 0.9% NS every 4 hours from enrollment until hospital discharge. The primary outcome was median LOS. Secondary outcomes were total adverse events, subdivided as clinical worsening and readmissions.

RESULTS: Patient characteristics were similar in groups. In intention-to-treat analysis, median LOS (interquartile range) of Hypertonic Saline and NS groups was 2.1 (1.2–4.6) vs 2.1 days (1.2–3.8), respectively, P = .73. We confirmed findings with per-protocol analysis, Hypertonic Saline and NS groups with 2.0 (1.3–3.3) and 2.0 days (1.2–3.0), respectively, P = .96. Seven-day readmission rate for Hypertonic Saline and NS groups were 4.3% and 3.1%, respectively, P = .77. Clinical worsening events were similar between groups (9% vs 8%, P = .97).

CONCLUSIONS: Among infants admitted to the hospital with bronchiolitis, treatment with nebulized 3% Hypertonic Saline compared with NS had no difference in LOS or 7-day readmission rates.

Abbreviations:

• AAP —American Academy of Pediatrics
• ED —emergency department
• HS —Hypertonic Saline
• IDS —Investigational Drug Services
• IQR —interquartile range
• LOS —length of stay
• NS —normal saline
• PRN —pro re nata
• RDAI —Respiratory Distress Assessment Instrument
• RSV —respiratory syncytial virus

What’s Known on This Subject

Bronchiolitis, the most frequent reason for hospitalization for infants younger than 1 year of age, has no proven treatments beyond supportive care. Although early studies suggested a potential benefit from 3% Hypertonic Saline, more recent studies have conflicting results.

What This Study Adds:

This prospective, randomized, double-blind, controlled trial in infants admitted with bronchiolitis (including patients with a history of previous wheeze) demonstrated no difference in length of stay between those who received Hypertonic Saline or normal saline without bronchodilators.

Bronchiolitis is the most frequent cause of hospital admission among infants in the United States.1–3 Total inpatient charges exceed $1.7 billion annually and mean length of stay (LOS) is 2.4 days.3 Treatment of this common and costly condition is mainly supportive, as previous studies suggest little benefit from bronchodilators,4,5 antibiotics,6 or steroids.7,8Some suggest nebulized 3% sodium chloride, Hypertonic Saline is helpful,9–14 but results are contradictory.15–17
Initial studies using Hypertonic Saline for infants hospitalized with bronchiolitis suggest decreased LOS and improved severity scores.9–14 Those studies differed from this study by excluding infants with previous wheeze, administering study medications with bronchodilators, or study subjects having much longer mean LOS.9,10,13,14 A 2013 Cochrane review of Hypertonic Saline in bronchiolitis referencing the aforementioned studies suggested 3% Hypertonic Saline given with bronchodilators may be considered a safe and effective treatment of infants with mild to moderate bronchiolitis.18 Subsequent studies15,17,19,20 suggest no benefit from Hypertonic Saline for inpatients, despite concomitant use of bronchodilators in all but one,19 which differed by being an open study. Results in Wu et al15 may be limited to a subpopulation with a diminished response to Hypertonic Saline, as the study began in the emergency department (ED), demonstrating decreased admission rate with Hypertonic Saline. The American Academy of Pediatrics (AAP) bronchiolitis guidelines updated in 2014 highlight the inconsistent findings regarding the effect of Hypertonic Saline on LOS but suggest Hypertonic Saline may be useful in infants admitted with bronchiolitis, particularly those with LOS >3 days.21 It is imperative to investigate the true efficacy and safety of Hypertonic Saline without confounding from bronchodilators, as unnecessary treatment can increase the financial burden of already costly bronchiolitis hospitalizations.
To understand the effects of Hypertonic Saline in bronchiolitis, we conducted a randomized controlled trial to test the hypothesis that Hypertonic Saline would decrease LOS in infants admitted with bronchiolitis without concomitant bronchodilators, including patients with previous wheeze.

Methods

Study Design

We conducted a prospective, randomized, double-blind, controlled parallel-group study of infants admitted with bronchiolitis, comparing 3% Hypertonic Saline with 0.9% normal saline (NS) from November 2011 through June 2014. The setting was an urban, tertiary care children’s hospital with 136 beds, >8800 total admissions per year, and ∼400 infants with bronchiolitis annually.
The principal investigator educated all physicians regarding the 2006 AAP definition of bronchiolitis: “a constellation of clinical symptoms and signs including a viral upper respiratory prodrome followed by increased respiratory effort and wheezing in children less than 2 years old.”22 The attending physician of record made the diagnosis. The ED physician–determined admission criteria included elements such as hypoxia (oxygen saturation <92% while awake), dehydration or failure to tolerate oral intake, tachypnea, or moderate to severe retractions demonstrating respiratory distress; a respiratory score was not a standard determinant of hospitalization. Inclusion criterion was infants 12 hours after admission. On first interim review by the data safety and monitoring board, another exclusion criterion was added: patients previously enrolled within 72 hours before presentation. Before this amendment, 2 patients enrolled in the Hypertonic Saline group met this criterion; 1 reenrolled in the Hypertonic Saline group and 1 in the NS group. Study personnel interviewed parents/guardians by using a standardized eligibility questionnaire. After written informed consent, recruiters collected background and demographic information by using a standardized questionnaire, and Montefiore Medical Center Investigational Drug Services (IDS) randomized patients by using a computer-generated block randomization scheme (www.randomization.com, 1:1 allocation; random block sizes of 8). Composition of the study solutions prepared by IDS was not disclosed to medical personnel. Appearance and smell of the solutions were indistinguishable in sequentially numbered containers, with allocation concealed by IDS. The identity of study solutions and random assignment of patients by IDS to intervention group (3% Hypertonic Saline) or control group (0.9% NS) was blinded to all study subjects, parents/guardians, medical care providers, and investigators. Study patients received 4 mL nebulized study solution, either Hypertonic Saline or NS based on assigned group, every 4 hours while remaining hospitalized. Patients could receive study treatment every 2 hours pro re nata (PRN), with a maximum of 2 PRN treatments per 24-hour period. Administration was via a Misty Max 10 nebulizer (Carefusion, Redlands, CA) with a pediatric vinyl under-the-chin aerosol mask connected to a standard hospital pressurized wall oxygen unit at 5 L/min (output 0.252 ± 0.009 g/min; aerodynamic mass median diameter 2.21 ± 0.07 µm). Using the validated Respiratory Distress Assessment Instrument (RDAI),23 investigators assigned patients a score before and 30 minutes after the first study treatment. RDAI functioned as a safety measure given theoretical risk of bronchospasm with Hypertonic Saline,24 not as an outcome measure, given its variable clinical utility.25 If the RDAI score increased by ≥4 points, the patient received a bronchodilator to relieve bronchospasm and was withdrawn from the study (n = 1). At the time of study entry, we reviewed AAP guidelines regarding use of bronchodilator and systemic steroids with providers. We asked to withhold these treatments barring clinical deterioration during the study. In the event of clinical worsening, the provider had the option of giving an additional study treatment; if the provider felt bronchodilators or corticosteroids were indicated and administered one, the patient was withdrawn from the study (n = 8). Additional exit criteria included transfer to the PICU, and request of the parent/guardian. Providers sent rapid virologic testing for respiratory syncytial virus (RSV) A and B, and influenza A and B, per routine hospital practice and clinician judgment. If rapid tests were negative or inconclusive, most patients had polymerase chain reaction testing for RSV, influenza, parainfluenza type 1, 2, 3, metapneumovirus, rhinovirus, and adenovirus per hospital practice. The attending physician of record assessed patients daily, recorded any adverse events, and determined if the patient would remain hospitalized for a nonbronchiolitis-related indication altering LOS unless noted (eg, social concerns, other medical issues) (n = 5). Otherwise, patients could be discharged at any time of day as per hospital practice. The physician-determined discharge criteria included elements such as stable respiratory status without supplemental oxygen for at least 12 hours including with sleep, and sufficient oral intake to maintain hydration and urine output. One week after discharge, research personnel conducted a standardized phone interview of the parent/guardian to determine if the patient had ongoing respiratory issues or was readmitted to any hospital for any reason. This study was approved by the institutional review board of the Albert Einstein College of Medicine. A data safety and monitoring board reviewed interim data analyses throughout the study.

Outcomes

The primary outcome was LOS, defined as the time from first study treatment to the time of either the order for hospital discharge or meeting discharge criteria. This definition of LOS has greatest clinical relevance given variability of waiting times in the ED and the potential 12-hour window from time of admission to enrollment. A comparison LOS using admission order time to discharge order time was done for verification.
Secondary outcomes included total adverse events, subdivided as 7-day readmission rate and clinical worsening. We defined clinical worsening as transfer to the PICU, or bronchospasm within 30 minutes of a nebulized study treatment indicated by an increase/worsening of the RDAI23 of ≥4 points. We systematically monitored for unforeseen adverse events (n = 0) throughout hospitalization and with a phone call 1 week after discharge.

Sample Size
Based on expert judgment and similar to a previous US study,15 we determined a clinically meaningful decrease in LOS (primary outcome) to be 0.6 days. We estimated prestudy infant bronchiolitis LOS (time of triage presentation to departure time from the hospital) at 2.85 days based on hospital administrative data. For power analysis, we used PASS software (NCSS Statistical Software, Kaysville, UT) with 3 different types of adjustments (logistic, uniform, and normal distribution) and chose the most conservative to determine sample size. Based on these analyses, to achieve 80% power with a 2-tailed α = 0.05, we needed 105 patients in each group to identify a 0.6-day change in LOS. Initially, we estimated 10% attrition rate due to request by the parent/guardian, PICU transfer, or provider choice to use albuterol. During interim analysis, attrition rate approximated 20%, so we adjusted our target sample size to 126 patients per study group.

Statistical Analysis
We compared patient demographic and clinical characteristics between intervention and control groups by using χ2 tests for categorical variables and t test for continuous normally distributed variables. We analyzed continuous non-normally distributed variables with Wilcoxon rank-sum tests. All tests were 2-sided (α = 0.05).
Given non-normally distributed LOS, we analyzed the difference in primary outcome (median LOS) between intervention and control groups with the Wilcoxon rank-sum test in an intention-to-treat analysis. To verify these findings, we performed a per-protocol analysis to give the study medication the best chance of demonstrating effect if one existed. Using the same methods, we performed post hoc analyses of LOS for patients by RSV status, history of previous wheeze, prematurity, and study entry RDAI ≥4 or hypoxia. We performed analyses using Stata software version 12 (Stata Corp, College Station, TX).
We used Fisher’s exact test to compare proportions of readmissions and adverse events. We used Fisher’s exact test to compare between study groups the proportion of patients who received PRN study treatments and t test to compare mean change in RDAI score before and after initial study treatment.

Results

We assessed 765 patients for eligibility and enrolled 227 subjects (Fig 1). Analyses were all by original assigned groups. There were no statistical differences between intervention and control groups for demographics and patient characteristics (Table 1). Of subjects with a race “Other,” most parents/guardians identified the patient as Hispanic. Eighty of 190 patients who completed the study qualified as moderately ill based solely on RDAI score ≥4 on study entry. Additionally, of those with RDAI score <4, nearly half (49/110) were hypoxic, an element of clinical severity not evaluated in the RDAI score. A total of 37 patients withdrew from the study in the final per-protocol analysis (Fig 1); the proportion withdrawn was similar between intervention and control groups (17.6% vs 14.9%, respectively, P = .59) (Supplemental Table 5). Patient characteristics were similar between included and withdrawn patients, except for a higher proportion of patients with previous wheeze and prematurity in the withdrawn group (Table 2). We closed enrollment in the study after achieving a sample size of 227 patients because interim futility analysis indicated that neither arm could achieve superiority with further enrollment. Discussion
This prospective, randomized double-blind controlled trial comparing Hypertonic Saline with NS without bronchodilators in hospitalized children<12 months old, including those with previous wheeze, found no difference in LOS between groups. Additionally, Hypertonic Saline was equivalent to NS in terms of adverse events, including clinical worsening and 7-day readmission. These findings are similar to those in recent studies conducted both with and without concomitant bronchodilators in the United States15 and abroad,17,19 but contradict findings of several other published studies.9,11–14 Our results challenge the conclusions of the most recent Cochrane review of Hypertonic Saline,18 which suggests a potential benefit of Hypertonic Saline. We believe this is partly attributed to the 2 to 3 times longer average LOS in the latter studies9,11–14 than in the studies in the United States and India. It is possible that Hypertonic Saline has a greater effect when administered over a longer period of time than in an acute setting with a shorter LOS. A living systematic review incorporating studies since the Cochrane found overall decrease in LOS with Hypertonic Saline.26 However, subgroup analysis noted no difference in patients with LOS The Cochrane review of Hypertonic Saline,18 published while this study was enrolling, based recommendations on data from 1090 patients in 11 studies. Ours is the fifth negative study15,17,19,20 since, totaling data from 1114 patients, demonstrating Hypertonic Saline is not effective inpatient therapy for bronchiolitis. Our study did not use bronchodilators and included patients with previous wheeze. Although we did not find that Hypertonic Saline decreased LOS, we also did not find an increase in adverse events, including in a post hoc subgroup analysis (limited by being underpowered) of important subpopulations: those with prematurity, previous wheeze, RSV + bronchiolitis, or with study entry RDAI ≥4 or hypoxia. Despite previous concerns about administering Hypertonic Saline to patients with a history of prematurity or previous wheeze, this study demonstrates both overall and in subgroup analyses safety of Hypertonic Saline administration without bronchodilators. Of note, chronic lung disease was an exclusion criterion, which may confound interpretation of results of those with prematurity. Bronchiolitis admissions consume substantial US health care resources, surpassing $1.7 billion annually in charges.3 It is imperative that research focus on using effective treatments for these infants, minimizing bothersome interventions without benefit. Our negative study may help reduce use of Hypertonic Saline and thereby decrease hospitalization costs and unnecessary resource utilization. The primary limitation of this study was its single-center nature, raising the question of generalizability; however, LOS was comparable to other US studies on bronchiolitis,3,15 and our results may be applicable to other urban centers with a diverse patient population. Second, enrolling patients within a 12-hour window from time of admission rather than on presentation could influence duration of time patients are in the study, especially for patients with a shorter LOS. However, this or even longer enrollment time frames were used in most other Hypertonic Saline clinical trials.9–13 A third limitation is no minimum severity score for eligibility; instead, admission to the hospital indicated severity of illness. Additionally, 42% of those completing the study had an RDAI score ≥4, and of 110 patients with scores <4, 45% were hypoxic before enrollment. There is also no difference in LOS in post hoc analysis of the subgroup of patients with RDAI ≥4 or hypoxia. Furthermore, the validity of respiratory scores, including RDAI, as a predictor of respiratory distress, disposition, and LOS is questionable,25,27 suggesting a respiratory score may be a flawed study entry criterion. The 16% attrition rate might be considered a limitation; however, most withdrawals were for provider choice to use albuterol (not unexpected given our population’s high endemic prevalence of asthma) or for PICU transfer (anticipated given natural course of illness). Additionally, although the mode of delivery was standardized, the approach to nebulized treatment administration in infants resisting was not and variability in administration to crying infants was possible. This likely reflects the reality of practice using nebulization in hospitalized infants. Finally, NS was the control instead of no treatment because Hypertonic Saline lacks a true-blinded placebo. If NS reduces LOS in infants admitted with bronchiolitis, this may conservatively bias these results. Although a recent open study compares Hypertonic Saline to supportive care alone,19 for the purpose of blinding in this study and based on previous literature,9–15,17we used NS as a control. Additionally, although not a measure of cumulative effect of treatment, we found no difference in RDAI score before and after initial study treatment in the NS group, suggesting NS had no significant clinical effect.

Conclusions

This study found no difference in LOS in infants <1 year of age, including those with previous wheeze, admitted with bronchiolitis to a tertiary care center treated with nebulized Hypertonic Saline as compared with those treated with NS without bronchodilators. Hypertonic Saline is safe for use without adjunctive bronchodilators, including in those with a history of previous wheeze, as rates of adverse events were similar between study groups. Although including patients with previous wheeze may confound the diagnosis of bronchiolitis, a key finding from this study is that Hypertonic Saline did not provoke bronchospasm. Future research could investigate effectiveness of higher saline concentrations, NS compared with supportive care alone, scheduled versus on-demand treatments, or 3% Hypertonic Saline in the outpatient setting. This study suggests there is no utility for the routine use of 3% Hypertonic Saline alone in treating infants hospitalized with bronchiolitis as compared with NS. Acknowledgments

We thank the patients and families who participated in this study. We thank Brian Currie, MD, MPH, for his support of the study, and we acknowledge the support of Clemencia Solorzano, PharmD, and the IDS. We thank the members of our Data Safety and Monitoring Board: Tsoline Kojaoglanian, MD, Peter Cole, MD, Nathan Litman, MD, and Marina Resnick, MD. We are grateful to Mimi Kim, ScD, who provided the futility analysis and Jaeun Choi, PhD, who added statistical support. We are indebted to Nathan Litman, MD, for his enduring support. We thank Shawn Ralston, MA, MD, for her encouragement and manuscript review. We thank all of the physicians and nurses, both in the ED and on the inpatient units, for their support of and contributions to this study. We thank those study personnel who helped recruit patients: Bradley Clark, MD, May Chen, MD, Michelle Cheng, MD, Jessica May Gold, MD, Keith Hazelton, MD, PhD, Jessica Herstek, MD, Nicole Leone, MD, Nina Mbadiwe, MD, Courtney McNamara, MD, Sara Dennis Meskill, MD, Kara Poezhl, MD, Natalie Uy, MD, and particularly Rama Subramanian, MD. Bronx Center to Reduce and Eliminate Ethnic and racial Disparities (Bronx CREED) Translational services (grant P60MD000514) provided without charge translation of the consent form into Spanish.

Source: Alyssa H. Silver, Nora Esteban-Cruciani, Gabriella Azzarone, Lindsey C. Douglas, Diana S. Lee, Sheila Liewehr,Joanne M. Nazif, Ilir Agalliu, Susan Villegas, Hai Jung H. Rhim, Michael L. Rinke, Katherine O’Connor; Pediatrics, December 2015, VOLUME 136 / ISSUE 6